Breeding technology is developing, processes are becoming more precise and simpler
Paper chromatography
1974
Since the determination of the fatty acid pattern was time-consuming and costly, rapid methods for the detection of individual fatty acids are being developed, e.g. paper chromatography for erucic acid in canola. This method can be used to more quickly "screen" larger populations for selection for specific fatty acids.
Double haploid breeding
1987
Double haploid breeding (DH breeding) is used to genetically fix plant traits much more quickly. This ensures in the breeding process that the genetic makeup of each subsequent generation of this genotype is consistent and homogeneous. The double haploid technique is carried out in the laboratory in Thüle and improves the efficiency of oilseed rape breeding immensely.
Near-infrared reflectance spectroscopy
1992
Near-infrared reflectance spectroscopy (NIRS method) is introduced. With this method, the ingredients of seeds can be determined very quickly. The duration of a measurement ("scan") is usually less than one minute, and various constituents of a sample can be detected simultaneously. Thus, time saving is the main advantage of this test method compared to other methods. It is possible to evaluate a sufficiently large number of genotypes for breeding selection purposes without chemicals and without time-consuming analytical steps.
Gas chromatography
1993
First seed quality analysis by gas chromatography (GC). Oils extracted from plant seeds are transesterified from the triglyceride esters into methyl esters, followed by chromotographic separation and quantitative detection of the individual fatty acids. In this way, the fatty acid pattern of an oil can be determined.
Marker assisted genome analysis
1995
Marker assisted genome analysis – this technology is revolutionised plant breeding at DSV. It allows a deep look inside the plant. The strategic combination of breeding and gene pool work with the latest knowledge and methods of genome analysis enables an unprecedented level of information and selection accuracy in the development of trait-inheriting breeding lines for agriculture.
Genomic selection
1999
A further development of marker-assisted selection is genomic selection, which entered DSV laboratories in 1999. Many traits such as yield, quality and resistance are not only dependent on one gene but on many. For these, further analyses are necessary, for example association mapping (assignment of phenotypic and genetic traits) or quantitative trait loci mapping (identification of chromosome segments with an influence on the expression of the traits).